International Symposium on
Drylands Ecology and Human Security

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Assessment and Application of Microbial Diversity

Hemant J Purohit

Environmental Genomics Unit, National Environmental Engineering Research Institute, Nehru Marg, Nagpur, India
e-mail: hj_purohit@neeri.res.in


Abstract

In an ecosystem air, water and soil makes the basic resources through which the nutritional needs and food chain start for survival of life.  The management of our basic resources is the most challenging issue facing us today. Most of the available approaches to assess impact on an ecosystem usually majors the perturbances after the ecosystem has reached an irreversible stage. The microbial communities surviving in these niches are principle biological receptors for any insult these ecosystems faces. This paper suggests the approaches how the microbial communities in these areas could be assessed.  It also describes the option by which instead of restating the base line data how the efforts can be made so that the base line of the ecosystem could be maintain.

The integrated approach in this paper uses molecular tools to describe the bacterial diversity and uses bioinformatics tool to discuss the dynamics of community associated in the ecosystem.  The paper proposes the simple chemical analysis to arrive at essential biogeochemical cycles of that ecosystem and uses statistical tools to assess the level of variations progressing in the system.  A model study has been carried out at a pesticide and near by a refinery has been presented to show the utilities of the suggested approach.